cd14 microbeads cat 130 050 201 cologne germany (Miltenyi Biotec)

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Miltenyi Biotec cd14 microbeads cat 130 050 201 cologne germany
Cd14 Microbeads Cat 130 050 201 Cologne Germany, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 98/100, based on 4686 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/germany+cat/CD14+MicroBeads%2C+human/pm42050116-73-43-41
Average 98 stars, based on 4686 article reviews

cd14 microbeads cat 130 050 201 cologne germany - by Bioz Stars, 2026-10

98/100 stars

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Purification:

Article Title: Changes in protein fluxes and gene expression in non‐injured muscle tissue distant from an acute myotoxic injury in male mice
Article Snippet: The response to acute myotoxic injury requires stimulation of local repair mechanisms in the damaged tissue.. However, satellite cells inmuscle distant from acute injury have been reported to enter a functional state between quiescence and active proliferation.. Here, we asked whether protein flux rates are altered in muscle distant from acute local myotoxic injury and how they compare to Alec Bizieff is a recent PhD graduate from the Department of Nutritional Sciences and Toxicology at the University of California, Berkeley.

FACS:

Magnetic Cell Separation:

Isolation:

Article Title: Dendritic Cells Pulsed with HAM/TSP Exosomes Sensitize CD4 T Cells to Enhance HTLV-1 Infection, Induce Helper T-Cell Polarization, and Decrease Cytotoxic T-Cell Response
Article Snippet: The HTLV-1 matrix protein (p19) often associated with virus production was not detected in the exosome isolations from HTLV-1-infected cell lines, confirming that virus was not concentrated in high quantities during the isolation process [ ]. .. Utilizing the Pan-DC Enrichment Kit-human (Miltenyi Biotec, Bergisch Gladbach, Germany, Cat. 130-100-777), DCs (MDC1s, MDC2s, and PDCs) were isolated from the PBMCs of normal donors using the buffy coat (500 μL). ..

Article Title: T cell receptors that bind to mixed lineage leukemia (MLL)-specific phosphopeptides and methods of use thereof
Article Snippet: Human dendritic cells (DCs) and CD8+ T cells were isolated from healthy donor HLA-B*0702+ PBMCs (Cellular Technologies Ltd., Shaker Heights, OH). .. Briefly, CD14+ monocytes were isolated from the PBMCs by positive selection via magnetic separation using anti-CD14 microbeads according to the manufacturer's instructions (Miltenyi Biotech, Bergisch Gladbach, Germany, Cat. No.: 130-050-201) and cultivated in growth medium composed of CellGro® DC-medium (Cell Genix, Cat. No.: 20801-05500) supplemented with 5% human serum (Sigma, Cat. No.: H3667-100 mL), 1% Penicillin/Streptomycin (Amimed Direct, London. .. UK, Cat. No.: 4-01F00-H), 800 U/mL of GM-CSF (Miltenyi Biotech, Cat. No.: 130-095-372), and 10 ng/mL of IL-4 (Miltenyi Biotech, Cat. No.: 130-093-917) for three days to induce differentiation to DCs.

Article Title: Dendritic Cells Pulsed with HAM/TSP Exosomes Sensitize CD4 T Cells to Enhance HTLV-1 Infection, Induce Helper T-Cell Polarization, and Decrease Cytotoxic T-Cell Response.
Article Snippet: The HTLV-1 matrix protein (p19) often associated with virus production was not detected in the exosome isolations from HTLV-1-infected cell lines, confirming that virus was not concentrated in high quantities during the isolation process [35]. .. Utilizing the Pan-DC Enrichment Kit-human (Miltenyi Biotec, Bergisch Gladbach, Germany, Cat. 130-100-777), DCs (MDC1s, MDC2s, and PDCs) were isolated from the PBMCs of normal donors using the buffy coat (500 μL). ..

Selection:

Magnetic Beads:

Article Title: Endothelial characteristics of cardiac stem cell antigen-1 expressing cells and their relevance to right ventricular adaptation.
Article Snippet: A growing body of evidence suggest that the stem cell antigen-1 expressing (Sca-1+) cells in the heart may be the cardiac endothelial stem/progenitor cells.. Their endothelial cell (EC) functions, and their role in right ventricle (RV) physiology and pathophysiology of right heart failure (RHF) remains poorly defined.. This study investigated EC characteristics of rat cardiac Sca-1+ cells, assessed spatial distribution and studied changes in Sca-1+ cells during RV remodelling in monocrotaline (MCT) model of pulmonary hypertension and RV remodeling.

Cell Culture:

Plasmid Purification:

Article Title: An Efficient Electroporation Protocol Supporting In Vitro Studies of Oligodendrocyte Biology.
Article Snippet: .. • DMEM/F12 (ThermoFisher Scientific, Waltham, MA, USA, Cat. no.: 10565018) • Neurobasal medium (ThermoFisher Scientific, MA, USA, Cat. no.: 12348017) • Opti-MEM I Reduced Serum Medium (ThermoFisher Scientific, MA, USA, Cat. no.: 31985062) • Hanks’ Balanced Salt Solution without Calcium and Magnesium (HBSS(−)) (ThermoFisher Scientific, MA, USA, Cat. no.: 14175053) • HBSS with Calcium and Magnesium (HBSS(+)) (ThermoFisher Scientific, MA, USA, Cat. no.: 14025092) • Poly-L-Ornithine (PLO) (Sigma, St. Louis, MO, USA, Cat. no.: A-004-M) • Matrigel growth factor reduced (MG-GFR) (Corning, NY, USA, Cat. no.: 354230) • Trace Elements B (1000×) (Corning, NY, USA, Cat. no.: 25-022-CI) • B27 supplement (50×) (ThermoFisher Scientific, MA, USA, Cat. no.: 17504044) • Platelet-derived growth factor (PDGF-AA) (Funakoshi, Tokyo, Japan, Cat. no.: 100-13A) • Ciliary neurotrophic factor (CNTF) (Funakoshi, Tokyo, Japan, Cat. no.: 450-13) • Neurotrophin-3 (NT3) (Funakoshi, Tokyo, Japan, Cat. no.: 450-03) • Triiodothyronine (T3) (Sigma, MO, USA, Cat. no.: T6397) • Nerve growth factor (NGF) (Merck, Darmstadt, Germany, Cat. no.: 01-125) • 5-Fluoro-5′-deoxyuridine (FdU) (Sigma, MO, USA, Cat. no.: F8791) • N-Acetyl-L-cysteine (NAC) (Sigma, MO, USA, Cat. no.: A9165) • Isoflurane (FUJIFILM, Osaka, Japan, Cat. no.: 099-06571) • 4% Paraformaldehyde Phosphate Buffer Solution (4% PFA) (FUJIFILM, Osaka, Japan, Cat. no.: 161-20141) • Neural Tissue Dissociation Kit (P) (NTDK (P)) (Miltenyi Biotec, Bergisch Gladbach, Germany, Cat. no.: 130-092-628) • CD140a (PDGFRα) MicroBead kit (Miltenyi Biotec, Bergisch Gladbach, Germany, Cat. no.: 130-101-502) • Dead Cell Makeup Deep Red (DOJINDO, Kumamoto, Japan, Cat. no.: C556) • AA3-PLP/DM20 (PLP) antibody [24] • βIII-Tubulin antibody (Sigma, MO, USA, Cat. no.: T8578) • GFP antibody (Abcam, Cambridge, UK, Cat. no.: ab13970) • Olig2 antibody (Merck, Darmstadt, Germany, Cat. no.: MABN50) • PiggyBac Transposon Vector (Funakoshi, Tokyo, Japan, Cat. no.: PB530A-2) • Super PiggyBac Transposase Expression Vector (Funakoshi, Tokyo, Japan, Cat. no.: PB210PA-1) .. • MS columns (Miltenyi Biotec, Bergisch Gladbach, Germany, Cat. no.: 130-042-201) • MiniMACS Separator (Miltenyi Biotec, Bergisch Gladbach, Germany, Cat. no.: 130-042-102) • MACS MultiStand (Miltenyi Biotec, Bergisch Gladbach, Germany, Cat. no.: 130-042-303) • Super Electroporator NEPA21 Type II (NEPA21) (NEPAGENE, Chiba, Japan) • Nepa Electroporation Cuvettes 2 mm Gap (NEPAGENE, Chiba, Japan, Cat. no.: EC-002) • Cuvette chamber (NEPAGENE, Chiba, Japan, Cat. no.: CU500) • 13 mm cover glass (MATSUNAMI, Osaka, Japan, Cat. no.: C013001) • 4 well multi-dish (ThermoFisher Scientific, MA, USA, Cat. no.: 176740) • Pasteur pipette (AS ONE, Osaka, Japan, Cat. no.: 2-2045-01) • Mini Cell Strainer II 40 μm (Funakoshi, Tokyo, Japan, Cat. no.: HT-AMS-04002) • All-in-one fluorescence microscopes (KEYENCE, Osaka, Japan, Cat. no.: BZ-X810)

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